Yeast Lysis Buffer plus Zymolase 1 kit

The Yeast Cell Lysis kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast lysis buffer is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. 

Yeast Lysis Buffer is based on organic buffering agents and utilizes a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into the Yeast Lysis Buffer. 

The Yeast lysis buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works with both Gram positive and negative bacteria. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. The Yeast Lysis Buffer kit eliminates the need for laborious glass bead lysis of yeast cells. 

This kit is suitable for processing 100 x 50 µl yeast cell pellet preparations. Yeast Lysis Buffer buffer is also available separately for further downstream processing.